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Description
The Glutamate Antibody was raised to glutamate coupled to KLH with glutaraldehyde. The antibody produces strong labeling of glutamate at dilutions of 1/2,000 - 1/4,000 using biotin-streptavidin/HRP technique. Glutamate tissue staining is completely eliminated by preincubation with glutamate conjugate at concentrations of 100 μg conjugate per mL of diluted antiserum. Aspartate and glutamine conjugates could not significantly inhibit tissue staining. The following amino acids were tested for cross reactivity using an enzyme-linked immunoassay method. Wells were coated with Glu-Glut-Btg at 1 μg per mL. Amino acids and conjugate were added at concentrations from 10 μg to 1 ng per mL. The Glutamate antibody was added to wells at 1 μg per mL. These amino acids were found to have cross reactivity at less than 1%: Beta-alanine, L-alanine, L-aspartic acid, L-glutamic acid, Glycine, Taurine and L-tyrosine.