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Description
Antibodies can be supplied as purified immunoglobulin on request, although most antibodies are available from stock. IgG antibodies are purified by affinity chromatography on a protein-G Sepharose column in an FPLC system. IgM antibodies are purified by a variety of different techniques including affinity chromatography (using an anti-mouse IgM column), ion exchange chromatography followed by size exclusion chromatography on Sephacryl S-300 or more recently using a capture select method. Unfortunately, not one single method can be applied for IgM purification as for each different IgM, the yields vary with each technique. The methods have to be optimised for each antibody. Purity of antibodies is checked by non-reducing SDS polyacrylamide gel electrophoresis, ELISA, serological methods or flow cytometry. Purified antibodies conjugated to either FITC, R-phycoerythrin (R-PE) or Biotin have been made available since 1994. Other conjugations can be requested on an individual basis as we have conjugated antibodies to peroxidase and Alexa Fluor 488. Again, various QC tests are carried out to ensure that the products are active and specific. Culture supernatants, purified antibodies and conjugated antibodies can be produced on a large scale according to GLP and can be sold on a collaborative or commercial basis. Two FITC conjugated antibodies have been CE marked. We have the capacity and skills to produce both Fab and F(ab)2 fragments but this is only carried out on an individual basis following communications with the customer.