Purity >98% (NMR, HPLC). A-769662 is a potent and reversible AMP-activated protein kinase (AMPK) allosteric activator. Activates AMPK by binding to the b and g subunits and not the AMP binding site. EC50=0.8 mM for purified rat liver AMPK and inhibits fatty acid biosynthesis in rat hepatocytes IC50=3.2 mM. A-769662 inhibits the rate of whole-body fatty acid oxidation, decreases body weight gain, reduces plasma and liver triglycerides and lowers plasma glucose in ob/ob mice. Inhibits adipocyte differentiation. Prevents transcriptional activation of Oct4 and establishes a metabolic barrier to induced pluripotent stem cell reprogramming. A-769662 synergizes with AICAR. Cell permeable.