Purity 96-99% (HPLC). Description: AA-dUTP (5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate) is supplied as a 10 mM aqueous solution at pH 7.0. Purity ≥ 96%.
Size
1 umol
Concentration
1 mumol (100 mul of 10 mM solution).
Applications
AA-dUTP can be used for enzymatic indirect non-radioactive labeling of DNA in PCR, nick-translation, cDNA synthesis or primer extension reactions. It can be incorporated into DNA using Reverse Transcriptases, Taq DNA Polymerase, and Klenow Fragment. The resulting aminoallyl-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten, resulting in a uniform and high density labeling of DNA.