|The assay is based on the sandwich ELISA technique. A monoclonal antibody specific for a conformational epitope on assembled AAV 5 capsids is coated onto microtiter strips and is used to capture AAV 5 particles from the specimen. Captured AAV 5 particles are detected in two steps. First a biotin conjugated monoclonal antibody to AAV 5 is bound to the immune complex. In the second step streptavidin peroxidase conjugate reacts with the biotin molecules. Addition of substrate solution results in a