Rat IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Rat IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum and anti-Rat IgM (μ chain specific). No reaction was observed against anti-Rat IgG F(c). Some light chain cross-reactivity will occur with anti-Rat IgG.
Size
1 mg
Concentration
2.01 mg/mL
Applications
ELISA, Immunohistochemistry, Western Blot. Rat IgM whole molecule can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.