ELISA, Immunoprecipitation, Western Blot. Anti Adenosine Deaminase antibody is suitable for ELISA, Western Blot and Immunoprecipitation applications. Anti-ADENOSINE DEAMINASE antibody has been assayed against 1.0 ug of Adenosine Deaminase [Calf Spleen] by immunoblot using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and DAB as a substrate at room temperature. A working dilution of 1:8,000 to 1:40,000 is suggested for this product.
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Description
Anti-ADENOSINE DEAMINASE antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen]. Cross reactivity against Adenosine Deaminase from other tissues and species may occur but have not been specifically determined.