Recombinant full-length human 14-3-3α/β was expressed in E.coli cells using an N-terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
Size
50 μg
Concentration
0.2 μg/μL
Applications
Western Blot. 14-3-3 alpha/beta Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. 14-3-3 alpha/beta Protein is suitable for use in Western Blot. Expect a band approximately ~54 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Other Names
14-3-3 alpha/beta, YWHAB, HS1, GW128, KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1