Bulk OEM Reagents (Reagent 1/ Reagent 2) for automated chemistry analyzers. Beta Blockers are also called beta-adrenergic blocking agents, beta antagonists, and beta-adrenergic antagonists. This group of drugs is used to treat angina, heart failure, migraines, high blood pressure, anxiety, and tremors. The major Beta Blockers include Atenolol, Metoprolol, Propranolol, Pindolol, and Bisoprolol. Insurance testing necessitates an accurate and reliable means for detection of Beta Blockers in urine. A high throughput detection method with high sensitivity and specificity is presented herein. Beta Blocker Assay provides a convenient and efficient method to detect the presence of multiple Beta Blocker compounds in human urine.