Bulk OEM Reagents (Reagent 1/ Reagent 2) for automated chemistry analyzers. 17-Hydroxycorticosteroids (17-OHCS) are metabolites of cortisol and other adrenocorticotropic hormones (ACTHs) found in urine. Measuring free cortisol levels aids with diagnosis of hypothalamic, pituitary, or adrenal malfunction. Elevated 17-OHCS concentrations may indicate Cushing's syndrome, adrenal cancer, hyperpituitarism, eclampsia, hyperthyroidism, while abnormally low concentrations may aid in diagnosing Addison's disease, adrenogenital syndrome, hypothyroidism, and hypopituitarism. 17-OHCS is not a standalone test, it is usually performed in combination with other tests. Standard disease screening procedures necessitate an accurate and reliable means for the measurement of 17-Hydroxycorticosteroids concentration. Due to the number of people affected by abnormal levels of cortisol metabolites; a high throughput detection method with high sensitivity and specificity is presented herein. 17-Hydroxycorticosteroids Assay provides a convenient and efficient method to monitor the metabolites concentration in urine.