Bulk OEM Reagents (Reagent 1/ Reagent 2) for automated chemistry analyzers. 17-Ketosteroids Assay is used to monitor androgen metabolites in urine. Androgens are a group of sex hormones and their metabolites characterized by a ketone in the 17 position of the steroid molecule. Some examples of 17-Ketosteroids include androsterone, androstanedion, dehydroepiandrosterone, epiandrosterone, and epietiocholanone. Elevated 17-Hydroxycorticosteroids concentrations may indicate Cushing syndrome, imbalance of sex hormones in females (polycystic ovary syndrome), ovarian cancer, and testicular cancer in men while abnormally low concentrations may aid in diagnosing Addison disease, kidney damage, and hypopituitarism. Standard disease screening procedures necessitate an accurate and reliable means to measure 17-Ketosteroids concentration in urine. A high throughput detection method with high sensitivity and specificity is presented herein. 17-Ketosteroids Assay provides a convenient and efficient method to monitor androgen metabolites in urine.