Bulk OEM Reagents (Reagent 1/ Reagent 2) for automated chemistry analyzers. Lp-PLA2, also known as platelet-activating factor acetylhydrolase (PAF-AH), is a phospholipase A2 enzyme produced by inflammatory cells. Lp-PLA2 is involved in the development of atherosclerosis and serves a specific marker of cardiac disease. Studies found that Lp-PLA2 levels are positively correlated with increased risk of coronary disease, stroke, and mortality. A high throughput detection method with high sensitivity and specificity is presented herein. Lp-PLA2 Activity Assay provides a convenient and efficient method to measure Lp-PLA2 activity in human serum.