The Cortisone CLIA kit is designed to quantitatively measure Cortisone present in extracted dried fecal samples, urine, saliva, and serum samples. This kit measures total cortisone in serum and plasma and in extracted fecal samples. A cortisone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a white microtiter plate coated with an antibody to capture rabbit antibodies. A cortisone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to cortisone to each well. After a two hour incubation the plate is washed and the chemiluminescent substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate to produce light. The generated light is detected in a microtiter plate reader capable of reading luminescence. The concentration of the cortisone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers. Sandwich CLIA (Chemiluminescent Immunoassay). Intra Assay Precision: Three human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Cortisone concentrations were: Sample 1- 7902.0 pg/mL, 7.9% CV Sample 2- 596.1 pg/mL, 5.7% CV Sample 3- 234.0 pg/mL, 10.1% CV Inter Assay Precision: Three human samples were diluted with Assay Buffer and run in duplicates in thirteen assays run over multiple days by three operators. The mean and precision of the calculated Cortisone concentrations were: Sample 1- 7904.0 pg/mL, 10.0% CV Sample 2- 662.7 pg/mL, 11.1% CV Sample 3- 262.9 pg/mL, 12.9% CV. Range: 78.1 - 20,000 pg/ml. Sensitivity: 10.6 pg/ml. Time: 2 hours.
Size
96 well
Concentration
n/a
Applications
CLIA kit used to quantitatively measure the cortisone present in samples. RUO