The Creatinine Serum Detection Kit is designed to quantitatively measure creatinine present in serum samples. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or samples are pipetted into a clear microtiter plate. An assay diluent is added to all standards, controls and samples. The color generating reaction is initiated with the StressXpress? Creatinine Reagent, which is pipetted into each well. The assay utilizes a kinetic absorbance method to overcome interference by colored compounds in serum. The absorbance of the colored product is read after 1 minute in a microtiter plate reader capable of measuring 490nm wavelength. At 30 minutes the optical density is read again. The concentration of creatinine is calculated using the delta of the optical density readings at 30 and 1 minute compared to the curve generated from the standards, or by using the Excel worksheet available for free download at our web site. The Jaffe reaction used in this kit has been modified to read creatinine levels in serum,8. Direct Quantitative Assay. Species Reactivity: human, mouse, rabbit, rat, sheep. Intra Assay Precision: Three human serum samples were run in replicates of 20 in an assay. The mean and precision of the calculated creatinine concentrations were: Sample 1- 0.99 mg/dL, 7.9% CV Sample 2- 1.50 mg/dL, 6.3% CV Sample 3- 3.82 mg/dL, 4.5% CV Inter Assay Precision: Three human serum samples were run in duplicates in 19 assays run over two years by four operators. The mean and precision of the calculated creatinine concentrations were: Sample 1- 0.91 mg/dL, 9.6% CV Sample 2- 1.26 mg/dL, 7.3% CV Sample 3- 3.51 mg/dL, 8.0% CV. Range: 0.5 - 4 mg/dl. Sensitivity: 0.081 mg/dl.
Size
2 x 96 well
Concentration
n/a
Applications
Colorimetric assay used to measure creatinine in samples. RUO