Colorimetric detection of 3,5-dibromotyrosine. Competitive ELISA (Enzyme-linked Immunosorbent Assay). Sample Types: plasma, urine. Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the Dibromo-tyrosine ELISA has been determined to be < 10%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays; the inter-assay coefficient of variation of the Dibromo-tyrosine ELISA has been determined to be < 10%. Range: 0.078 - 5 μg/mL. Sensitivity: 0.04 μg/ml. Time: 1 hour. No detectable cross-reactivity was observed at assay range 0.078 μg/mL to 5 μg/mL for tyrosine, di-tyrosine, 3-nitro-tyrosine or 3-bromo-tyrosine.
Size
5 x 96 well
Concentration
n/a
Applications
ELISA kit used to quantify free and protein-bound 3,5-dibromotyrosine in samples. RUO