Cell proliferation assay plays a significant role in cytotoxicity and many other areas of cell biology. A number of methods have been developed for the determination of cell proliferation. Since Fluorescent Cell Proliferation Assay enables accurate counts of cells for a wide range of cell concentrations and experimental conditions, this assay have been the most widely used in the quantification of cell proliferation among all of these methods. High throughput screening and high sensitivity can also be achieved in the study of cell proliferation.