The absorbance is measured by a microplate-reader, set at 450 nm or 492 nm with 620 nm as a reference. The reference absorbance at 620 nm (or any wavelength between 620-690 nm) is used to correct for nonspecific background values, caused by cell debris, fingerprints, or other potential interferences. However, the reference may be omitted without significant changes in the accuracy of the assay. Sample Types: serum, plasma. Range: 0 - 10 μg/ml. Sensitivity: 0.05 μg/ml. Time: 3.5 hours ? The Eagle Biosciences MDA Oxidized LDL ELISA Assay kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human MDA Oxidized LDL in serum or plasma. The MDA Oxidized LDL ELISA Assay kit is manufactured by Biomedica. The MDA Oxidized LDL ELISA Assay kit is for research use only and not to be used in diagnostic procedures.