Functionally active Alpha-2-antiplasmin present in plasma reacts with plasmin that has been coated and dried on a microtiter plate. Latent or complexed a-2-antiplasmin will not bind to the plate or be detected. Unbound a-2-antiplasmin is removed by washing and an anti-a-2-antiplasmin primary antibody is added. Excess primary antibody is removed by washing. The bound antibody, which is proportional to the original active a-2-antiplasmin present in the samples, is then reacted with the horseradish peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate solution is then used for color development at 450 nm. The amount of color development is directly proportional to the concentration of active a-2-antiplasmin in the sample. Sample Types: plasma. Range: 0.025 - 10 ng/ml. Sensitivity: 0.025 ng/ml. Time: 1.5 hours ? This Mouse a-2-antiplasmin (Alpha2-AP) Active ELISA is for the quantitative determination of active a-2-antiplasmin in mouse plasma. a-2-antiplasmin (Alpha2-AP) is the major circulating inhibitor of plasmin, and it has a role in the regulation of intravascular fibrinolysis1,2. Decreased levels of a-2-antiplasmin may play an important role in the increased capacity of the fibrinolytic function and may be beneficial in the treatment of thrombotic diseases, acute pulmonary embolism, and hepatic repair