FITkit Hev b 6.02 test is based on the immunoenzymetric assay technique. Microtiter wells are coated with Hev b 6.02-specific monoclonal capture antibody that during the first incubation binds Hev b 6.02 molecules from the sample extract. After incubation, unbound material is removed by washing the wells. During the second incubation, horseradish peroxidase (HRP) labelled Hev b 6.02-specific monoclonal detection antibody binds to a different epitope of Hev b 6.02 molecules. As such, the two antibodies are a matched pair that forms a ``sandwich'' around the target Hev b 6.02 allergen. After washing, HRP substrate is added and the intensity of the colour produced is directly proportional to the Hev b 6.02. Sample Types: natural rubber products. Range: 10 -1000 μg/L. Sensitivity: 0.1 μg/L. Time: 2 horus ? The Eagle Biosciences FITkit? Hev b 6.02 test is the first commercial quantitative test to measure Hev b 6.02 immunologically in NRL products. By the use of specific monoclonal antibodies, sensitivity and specificity stay high even in the presence of other proteins or chemical substances derived from the manufacturing process of Natural Rubber Products (NRL) products.