Anti-GAD ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of autoantibodies to glutamic acid decarboxylase (GAD65 Abs) in human serum. The Anti-GAD ELISA Assay Kit uses the ability of GAD65 Abs acting divalently and forming a bridge between immobilized GAD65 and liquid-phase GAD65-Biotin. In the first step GAD65 Ab from the sample bind to GAD65 coated on the microtiter plate. In a second step GAD65-Biotin binds to this complex. The bound GAD65-Biotin correlates with the amount of GAD65 Abs in patient's serum. Unbound GAD65-Biotin is removed by washing. The bound GAD65-Biotin could be quantified by addition of Streptavidin- peroxidase and a colorogenic substrate (TMB) and reading the optical density (OD) at 450 nm. Sample Types: serum. Range: 5 - 250 IU/ml. Sensitivity: 0.8 IU/ml. Time: 3 hours ? The Eagle Biosciences Anti GAD ELISA Assay Kit (Enzyme immunoassay) is for the determination of autoantibodies to Glutamic Acid Decarboxylase (GAD65 Abs) in human serum. The Eagle Biosciences Anti GAD ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.