Anti IFI16 ELISA Assay Kit is based on the binding of antibodies present in calibrators, controls or pre-diluted samples to the IFI16 Antigens coated on the inner surface of the microplate wells. After 30 minutes incubation the microplate is washed with wash buffer to remove the non-reactive serum components. An anti-human-IgG horseradish peroxidase conjugate solution recognizes IgG class antibodies bound to the immobilized Antigenss. After a 30 minutes incubation excess enzyme conjugate, which is not specifically bound is washed away with wash buffer. Sample Types: serum, plasma. Range: 50 - 80 AU/ml. Sensitivity: 1.16 AU/ml. Time: 1.5 hours ? Anti IFI16 ELISA Assay Kit is a semi-quantitative, enzyme-linked immunosorbent assay (ELISA) for the detection of IgG class antibodies directed against the IFI16 Antigens in human serum or plasma. Systemic autoimmune disease is characterized by the presence of circulating autoantibodies. Systemic Sclerosis (SSc) is an autoimmune multisystem connective tissue disorder characterized by inflammatory and fibrotic processes, affecting the skin, blood vessels and internal organs. The two widely recognised subsets of SSc are limited cutaneous SSc (lcSSc) and diffuse cutaneous SSc (dcSSc). These two forms of SSc present divergent patterns of internal organ involvement, clinical course and autoantibody profiles, as defined by the Le Roy criteria (LeRoy et al. 2001). SSc is characterised by the occurrence of antinuclear autoantibodies. Anti-centromere (ACA), anti-DNA topoisomerase-I (anti-Scl70) are the most common autoantibodies and, along with anti-RNA polymerases (RNAP I-III), are specific to SSc.
Size
1 x 96 well
Concentration
n/a
Applications
RUO
Other Names
Gamma-interferon-inducible protein Ifi-16 (Ifi-16), interferon-inducible myeloid differentiation transcriptional activator