The Anti-LKM1 (Liver-Kidney Microsomes) ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of IgG antibodies to cytochrome P450 IID6. The antibodies of the standards, controls, and diluted samples react with human recombinant cytochrome P450 IID6 immobilized on the solid phase of microtiter plates. The use of highly purified sample recombinant cytochrome P450 IID6 guarantees the specific binding of LKM-1 antibodies of the specimen under investigation. Following an incubation period of 30 min at room temperature (RT), unbound serum components are removed by a wash step. Sample Types: serum, plasma. Range: 3 -300 U/ml. Sensitivity: 3 U/ml. Time: 90 minutes ? The Anti-LKM1 (Liver-Kidney Microsomes) ELISA Assay Kit is used for the quantitative deter mination of IgG antibodies to cytochrome P450 IID6 in human serum or plasma.