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Description
This antibody was cross-adsorbed to unphosphorylated βIII-Tubulin (Ser-172) peptide before affinity purification using phospho-βIII-Tubulin (Ser-172) peptide (without carrier). The antibody detects a 50 kDa protein corresponding to the molecular mass of β-Tubulin on SDS-PAGE immunoblots of purified brain tubulin phosphorylated with ERK2 kinase.
Immunogen
Phospho-βIII-Tubulin (Ser-172) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 172 of human βIII-Tubulin. This sequence is identical to similar regions in βI, βII, and βIII-Tubulin isotypes, and is well conserved in tubulins from most eukaryotic species.