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Description
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-c-Abl (Tyr-245) peptide before affinity purification using phospho-c-Abl (Tyr-412) peptide. On SDS-PAGE immunoblots of K-562 treated with pervanadate the antibody detects a 145 kDa protein corresponding to c-Abl and a 210 kDa band corresponding to BCR-Abl.
Immunogen
Phospho-c-Abl (Tyr-412) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 412 of human c-Abl. This peptide sequence has high homology to the conserved site in rat and mouse c-Abl, as well as in viral Abl and BCR-Abl fusion protein.