Myosin IIA Heavy Chain (Ser-1803), phospho-specific
Clone
polyclonal
Host Species
Rabbit
Reactive Species
human, rat, mouse, chicken
Isotype
n/a
Format
affinity purified
Size
100 μl
Concentration
n/a
Applications
WB, ELISA
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Description
This antibody was cross-adsorbed to unphosphorylated Myosin IIA Heavy Chain (Ser-1803) peptide before affinity purification using phospho-Myosin IIA Heavy Chain (Ser-1803) peptide (without carrier). The antibody detects a 200 kDa protein corresponding to Myosin IIA Heavy Chain on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A. This reactivity is removed after treatment with lambda phosphatase.
Immunogen
Phospho-Myosin IIA Heavy Chain (Ser-1803) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1803 in human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse myosin IIA heavy chain, and the site has some homology to the conserved sites in nonmuscle myosin IIB and smooth muscle myosin heavy chains.