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Description
Clone M016 mouse monoclonal antibody detects a 40 kDa protein on SDS-PAGE Native immunoblots of human A431, LNCaP, MeWo, MDA-MB-231, and MCF7 cells. This antibody does not detect denatured Na+/K+ ATPase β3 subunit. In addition, mass spectrometry analysis of immunoprecipitates using NM0161 in human A431 cell lysates confirms that this antibody only detects Na+/K+ ATPase β3 protein.The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the β3 subunit on live cells.
Immunogen
Clone (M016) was generated from a proprietary antigen related to the native human Na+/K+ ATPase β3 subunit expressed in A431 epidermoid carcinoma cell line.