Human IGF-II, recombinant protein (Animal/Media Grade)
Description
Product Specifications Human IGF-II: Insulin-like growth factor-II (IGF-II) is a 67 amino acid polypeptide that shares structural homology with insulin and IGF-I. IGF-II is widely distributed in many tissues throughout the body and acts on numerous cell types through the Type 1 IGF receptor. IGF-II binds specifically to the IGF-II / cation-independent mannose-6-phosphate receptor, as well as tothe family of IGF binding proteins, but the physiological function as a result of all these interactions has not been clearly established. Link to Sequence: Source: IGF-II is synthesized in E. coli using a patented expression system. GroPep scientists and engineers have produced Animal / Media grade IGF-II to provide a high quality reagent for use in studies on cellular and animal growth and for inclusion in cell culture medium.Purity:> 90% (as determined by HPLC and N-terminal sequence analysis)Molecular weight:7469 daltons - major peak - confirmed by Mass SpectrometryAppearance:White PowderN-terminal sequence analysis:5 residues > 90% single sequenceEndotoxin (LAL):<0.1 EU/ugBiological Activity:(stimulation of protein synthesis in L6 myoblasts) ED50 <150 ng/mlState:Dried from 0.1 M acetic acid under dry nitrogen at a slight vacuum (-25kPa)Reconstitution:Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g. <1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles.Storage/Stability: at least 2 years at 2-4°C (lyophilised).This is a Gropep specification. ibt is authorized distributor of GroPep.
Size
100 μg
Concentration
n/a
Applications
n/a
Other Names
insulin like growth factor-2, IGF-2, Somatomedin A, ILGF2, T3M-11-derived growth factor,