Product Specifications Biotin-(Leu24) IGF-I: Biotin- Leu24 IGF-I is prepared by a selective biotinylation procedure.As in the non-biotinylated analog, binding to the receptor is strongly reduced. Binding to IGFBP-1 to -5 is not substantially affected.Examples of applications of biotinylated IGF's can be seen in application note 4.Molecular Weight: 7956.5 Da. Formulation: Lyophilised from a solution of 0.5 ml TBS, pH 7.4, containing 10 μg of the biotinylated product . A preservative and a stabilising protein of non-mammalian origin are added to ensure stability of the reconstituted product. Recommended dilution: 1: 100 to 1:250 for western ligand blotting. Tested with our western ligand blotting kit reagents. Storage: Store refrigerated upon arrival. The product is stable at 4 °C for at least one year. Reconstitute with 0.5 ml water and store aliquots frozen at - 20 °C. Avoid repeated freeze-thawing cycles.