(Leu24) IGF-I, recombinant protein (Receptor Grade)
Description
Product Specifications (Leu24) IGF-I, recombinant protein: Human [Leu24]Insulin-like Growth Factor-I (IGF-I) is an analog of human IGF-I with a Leu substitution in place of Tyr at position 24. Compared to human IGF-I, human [Leu24]IGF-I has greatly reduced binding to the Type 1 IGF receptor and to the insulin receptor, but binding to acid-stripped human serum binding is unaltered. Consequently human [Leu24]IGF-I is an extremely useful analog of IGF-I for investigating IGF actions largely independent of binding to the Type 1 IGF receptor.
Bayne M.L. et al. (1990) J. Biol. Chem. 265, 15648-15652 Source: E,coli.Purity:> 95 % (by HPLC and N-terminal sequence analysis) Molecular Weight: 7599 daltons - confirmed by Mass Spectrometry N-terminal sequence analysis: 5 residues > 95 % single sequence Biological Activity:Type 1 IGF receptor binding assay: ED50 > 100 ng/mlIGF binding protein assay: ED50 < 10 ng/ml. Stimulation of protein synthesis in rat L6 myoblasts: ED50 < 100 ng/ml Endotoxin: < 0.1 EU/μg State and Appearance:Lyophilized white powder. Dried from 0.1 M acetic acid and stored under nitrogen at a slight vacuum.Storage/Stability:At least 2 years at 2 - 4°C (lyophilized). This is a GroPep specification. ibt is authorized distributor of GroPep.