Product Specifications Rat IGF-II: Rat Insulin-like Growth Factor-II is polypeptide of 67 amino acids, four of which differ from human IGF-II at positions 22, 32, 35 and 36, with the latter three changes occuring in the C-domain. Link to Sequence.
Size
20 μg
Concentration
n/a
Applications
n/a
Other Names
insulin like growth factor-2, IGF-2, Somatomedin A, ILGF2, T3M-11-derived growth factor,
Dull TJ et al (1986) Nature 310, 771-781.Source: Produced recombinantly in E. coliPurity: > 95% (as determined by HPLC and N-terninal sequence analysis Molecular weight: 7515 Da, confirmed by Mass SpectrometryAppearance:Lyophilized white powderN-terminal sequence analysis: 5 residues > 95% single sequenceEndotoxin (LAL): < 0.01 EU/μgBiological Activity: Rat IGF-II has a similar potency to human IGF-II for binding to the Type 2 IGF receptors on rat L6 myoblasts. Both ED50 values are approximately 3.8 ng/ml. Rat IGF-II and human IGF-II also exhibit a similar potency for the stimulation of protein synthesis in rat L6 myoblasts. In this assay both ED50 values are approximately 15 ng/ml.State:Dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum.Reconstitution:Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilution (e.g. < 1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles. Storage/Stability: At least 2 years at 2-4°C (lyophilized) This is a Gropep specification. ibt is authorized distributor of GroPep.