mouse, human, Saccharomyces cerevisiae, Arabidopsis thaliana, Caenorhabditis elegans, fruit fly (Drosophila melanogaster), Schizosaccharomyces pombe, African green monkey
Isotype
IgG1
Format
Protein A purified
Size
50ul
Concentration
0.8mg/ml
Applications
Flow Cyt, ICC/IF, ChIP, ChIP/Chip, WB, ELISA, IP
Reviews / Ratings
If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY.
Description
Mouse Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) [4H8] - ChIP Grade. Recognises the C-terminal repeat of the largest subunit of RNA polymerase II. It recognises both unphosphorylated and phosphorylated forms.
Immunogen
Synthetic peptide: YSPTSpPS (Human). The sequence is repeated multiple times in the C-terminal domain of RNA polymerase II
Other Names
DNA directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit, Polr2a, RNA pol II CTD, RNApolymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RPB1, RPB1_HUMAN
The HRPT2 tumor suppressor gene product parafibromin associates with human PAF1 and RNA polymerase Yart A et al. Cell Biol. 2005 Jun;25(12):5052-60 ChIP ; HumanPMID: 15923622 Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation Kristjuhan A, Wittschieben BO, Walker J, Roberts D, Cairns BR, Svejstrup JQ. ( Proc Natl Acad Sci U S A,2003) PUBMED Keren-Shaul H et al. Pre-mRNA splicing is a determinant of nucleosome organization. PLoS One 8:e53506 (2013). WB, ChIP ; Human PUBMED Schmucker AC et al. Distal Interleukin-1AY (IL-1AY) Response Element of Human Matrix Metalloproteinase-13 (MMP-13) Binds Activator Protein 1 (AP-1) Transcription Factors and Regulates Gene Expression. J Biol Chem 287:1189-97 (2012). ChIP ; Human PUBMED Davidson L et al. Co-transcriptional degradation of aberrant pre-mRNA by Xrn2. EMBO J 31:2566-78 (2012). ChIP PUBMED Harrigan JA et al. Replication stress induces 53BP1-containing OPT domains in G1 cells. J Cell Biol 193:97-108 (2011). ICC/IF ; Human PUBMED Chen X et al. Sequential changes at differentiation gene promoters as they become active in a stem cell lineage. Development 138:2441-50 (2011) PUBMED