The Ansh Labs' Free Rat/Mouse IGF-I ELISA kit is a quantitative one-step sandwich assay. There has been interest in the measurement of unbound IGF-I although the existence of a true unbound IGF (biologically active fraction) is controversial, pharmacokinetic studies indicate that a small % of plasma IGF-I is not associated with IGF-binding proteins. Unbound IGF-I, which has also been observed in saliva, may exert a tonic hypoglycemic effect that is inhibited by exogenous IGFBP-1 administration. The calibrators are traceable to World Health Organization IGF-I preparation NIBSC code 02/245, version 6.0. Small sample size of 10 uL. Wide dynamic range of 0.251 - 10.036 ng/mL. Sensitive to 0.1108 ng/mL. Single 1 hr incubation at room temperature. Research Use Only within the United States. Ansh Labs is an FDA registered manufacturer of diagnostic test kits, antibodies, antigens, and proteins.