human, mouse, rat, porcine, bovine, primate, rabbit, sheep
Isotype
n/a
Format
immunogen affinity purified
Size
0.025 ml
Concentration
1.0 mg/ml
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting. Immunohistochemistry-Frozen was reported in scientific literature. In Western blot this antibody recognizes a band at 67 kDa or larger representing isoform 1 of NOX4, and what appears to be a non-specific band 48 kDa. The observed band size may vary depending on sample type and glycosylation. In ICC/IF cytoplasmic and mitochondrial staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunoblotting reported in scientific literature (PMID 28152080).
Reviews / Ratings
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Description
The Nox4 Antibody from Novus Biologicals is a rabbit polyclonal antibody to Nox4. This antibody reacts with human, mouse, rat, porcine, bovine, primate, rabbit, sheep. The Nox4 Antibody has been validated for the following applications: Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting.
Immunogen
A synthetic peptide made to an internal region of the human NOX4 protein (between residues 100-200) [UniProt Q9NPH5].