human, mouse, rat, porcine, bovine, primate, rabbit, sheep
Isotype
n/a
Format
Alexa Fluor 488 conjugate
Size
0.1 ml
Concentration
n/a
Applications
Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. This NOX4 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry-paraffin embedded sections. Immunohistochemistry-Frozen was reported in scientific literature. In Western blot this antibody recognizes a band at 67 kDa or larger representing isoform 1 of NOX4, and what appears to be a non-specific band 48 kDa. The observed band size may vary depending on sample type and glycosylation. In ICC/IF cytoplasmic and mitochondrial staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reviews / Ratings
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Description
The Nox4 Antibody [Alexa Fluor? 488] from Novus Biologicals is a rabbit polyclonal antibody to Nox4. This antibody reacts with human, mouse, rat, porcine, bovine, primate, rabbit, sheep. The Nox4 Antibody [Alexa Fluor? 488] has been validated for the following applications: Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.
Immunogen
A synthetic peptide made to an internal region of the human NOX4 protein (between residues 100-200) [UniProt Q9NPH5].