Purity ≥ 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Recombinant Human NAP-2 is a non-glycosylated, Polypeptide chain containing 70 amino acids and having a molecular mass of 7609 Dalton. Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Glu-Leu-Arg-Cys Dimers and Aggregates: ≤ 1% as determined by silver-stained SDS-PAGE gel analysis. Biological Activity: NAP-2 is fully biologically active when compared to standard. The specific activity as determined by the ability of NAP-2 to chemoattract human neurotrophils using a concentration of 1-10ng/ml. Endotoxin: ≤ 0.1ng/ug (IEU/ug) of NAP-2. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a standard solution of NAP-2 as a Reference Standard. Reconstitution: Reconstitute the lyophilized NAP-2 in sterile 18Mω-cm H2O not less than 100ug/ml,