Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 5mM DTT . NELFE is a part of complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. It contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). Recombinant human NELFE protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
Size
100ug, 20ug, 4ug
Concentration
n/a
Applications
SDS-PAGE
Other Names
Negative elongation factor E, Negative elongation factor complex member E, D6S45, NELF-E, RD, RDBP, RDP