ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. Anti-Laminin biotin conjugated antibody is suitable for western blotting, IHC, IP and for ELISA and for use with streptavidin reporter reagents. Researchers should determine optimal titers for applications that are not stated below.
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Description
Anti-Laminin has been prepared by immunoaffinity chromatography using immobilized human placental laminin followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross-reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.
Immunogen
Non-reduced 400 kDa human laminin. Immunization occurred after purity assessment of native protein in non-reducing SDS-PAGE system.