ELISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot. This Anti-AKT affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user for IF and IHC. Expect a band approximately 55-60 kDa in size corresponding to AKT1 by western blotting in the appropriate cell lysate or extract. Cell Signaling, Neuroscience, Signal Transduction research.
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Description
This antibody is directed against AKT and reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse and chicken AKT.
Immunogen
Anti-AKT antibody was affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human AKT1 protein. A residue of cysteine was added to facilitate coupling.