ELISA, Flow Cytometry, Immunohistochemistry, Western Blot. Anti-AKT Antibodies are suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT proteins by western blotting in the appropriate cell lysate or extract. All 3 primary monoclonal antibodies reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required. This sampler kit consists of sample sizes of anti-AKT1, anti-AKT2 and anti-AKT3 mouse monoclonal antibody and HRP conjugated anti-Mouse IgG secondary antibody (p/n 200-301-I51S; 200-301-BR8S; 200-301-J36S and 610-403-C46S, respectively).
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Description
This primary antibody kit has been carefully chosen to recognize all 3 isoforms of AKT. Anti-AKT1 antibody is directed against human AKT1. Anti-AKT2 antibody is directed against human AKT2. Anti-AKT3 antibody is directed against human AKT3. The antibodies detect both unphosphorylated and phosphorylated forms of the protein. The antibodies were purified from ascites by Protein A chromatography.
Immunogen
All Anti-AKT isoform specific Antibodies were produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1, AKT2 or AKT3 protein.
Other Names
AKT isoform antibody kit, AKT-1, AKT-2, AKT-3, Protein kinase Akt antibody, Protein kinase B antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, antibody sampler,