ELISA, Flow Cytometry, Immunohistochemistry, Western Blot. This antibody is phospho specific for pS473 and is suitable for western blotting, immunohistochemistry (formalin-fixed paraffin-embedded sections), immunofluorescence and ELISA. By immunoblot a single band of the expected apparent molecular weight is observed. For immunohistochemistry no pre-treatment of sample is required.
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Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated human AKTpS473. Minimal reactivity occurs against non-phosphorylated AKT. Reactivity against AKT from other species may occur but has not yet been tested.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a C-terminus region near aa 460-480 of human, mouse, rat and chicken AKT proteins conjugated to KLH.
Henle SJ, Wang G, Liang E, Wu M, Poo MM, Henley JR. (2011) Asymmetric PI(3,4,5)P3 and Akt signaling mediates chemotaxis of axonal growth cones. J Neurosci. 2011 May 11;31(19):7016-27. doi: 10.1523/JNEUROSCI.0216-11.2011.