ELISA, Immunohistochemistry, Western Blot. Anti-AIF Antibody has been tested for use in ELISA, western blotting and immunohistochemistry. K562 cell lysate, as well as rat and mouse heart tissue lysates, can be used as positive controls in western blotting, and a band at approximately 67 kDa is expected. Specific conditions for reactivity should be optimized by the end user.
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Description
Anti-AIF Antibody is directed against human AIF protein. The product was purified from monospecific antiserum by DEAE ion exchange chromatography. A BLAST analysis was used to suggest cross-reactivity with AIF protein from mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Immunogen
AIF Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at an internal region of human AIF protein.