Sapphire Prestained Protein Standard 10-180kDa contains approximately 0.1-0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Size
500 μL
Concentration
n/a
Applications
Sapphire Prestained Protein Standard 10-180kDa is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading. Suggested load volumes: 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively - 2-3 μl per well for general Western blot transferring. - Apply more for thicker (>1.5 mm) or larger gel.
Other Names
Protein ladder, Protein marker, Protein standard, Protein molecular weight standard, Molecular weight marker, Protein molecular weight ladder, Prestained molecular weight marker, Prestained molecular weight ladder, Prestained protein ladder, Protein marker