Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Sepharose? Protein A can be used for immunoprecipitation and purification of monoclonal antibodies.
Size
2 mL
Concentration
6.0 mg/cc
Applications
Immunoprecipitation. Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 μL per immunoprecipitation reaction.
Other Names
ProA, Staphylococcus A protein, Sepharose Protein A