The Human NGF / NGFB ELISA Pair Set is for the quantitative determination of Human NGF / NGFB.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human NGF / NGFB coated on a 96-well plate. Standards and samples are added to the wells, and any Human NGF / NGFB present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human NGF / NGFB monoclonal antibody is then added, producing an antibody-antigen-antibody sandwich. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human NGF / NGFB present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.