A fixable membrane probe for high resolution microscopy. The membrane-binding fluorophore-cysteine-lysine-palmtoyl group (mCLING) is a new probe that selectively binds to the plasma membrane. It is taken up during endocytosis and, in contrast to conventional membrane dyes, remains attached to membranes after fixation and permeabilization and can therefore be combined with immunostaining and super-resolution microscopy. mCLING was used so far in mammalian-cultured cells, yeast, bacteria, primary cultured neurons, Drosophila melanogaster larval neuromuscular junctions, and mammalian tissue.