Equine Infectious Anemia Virus Antibody Test Kit, ELISA v2
Description
This Equine Infectious Anemia Virus Antibody Test Kit, ELISA is an enzyme-linked immunosorbent assay (ELISA). It is unusual in that it is neither an indirect nor a competitive ELISA. Rather, we refer to it as a modified sandwich ELISA. This format has the species agnosticism of a cELISA, and possibly improves on the inherent specificity of a cELISA, without being hampered by the single-epitope limitations of a cELISA. Equine serum is incubated in the EIAV p26 Antigen-Coated Plates and removed by dumping. Bound antibody is detected by adding Antigen-Peroxidase Conjugate (purified recombinant EIAV p26 antigen conjugated to HRP; the same antigen used to coat wells) followed by washing and Substrate Solution addition. After incubation, Stop Solution is added, changing the color of the Positive Control and any positive serum samples from blue to yellow. The results can be read using a microplate reader or visually by comparison with the Positive Control. This test for antibodies to EIAV is rapid (~35 min), yet provides high specificity and sensitivity.