Glial Fibrillary Acidic Protein (GFAP) Recombinant, Human
Description
Purity ≥95%. Endotoxin: ≤1EU/ug (LAL). Recombinant protein corresponding to aa254-374 with two N-terminal Tags, His-Tag and S-Tag from human GFAP, expressed in E. coli (P14136) Amino Acid Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-EEWYRSKF ADLTDAAARN AELLRQAKHE ANDYRRQLQS LTCDLESLRG TNESLERQMR EQEERHVREA ASYQEALARL EEEGQSLKDE MARHLQEYQD LLNVKLALDI EIATYRKLLE GEE Predicted Molecular Weight: 20kD Accurate Molecular Weight: 25kD as determined by SDS-PAGE reducing conditions Note: The possible reasons that the actual band size differs from the predicted are as follows: 1. Splice variants: Alternative splicing may create different sized proteins from the same gene 2. Relative charge: The composition of amino acids may affect the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form. 5. Polymerizati