The Eagle Biosciences ANAC-C (PR3) hs ELISA Assay Kit is an Enzyme Immunoassay for the quantification of IgG auto-antibodies against Proteinase 3 in serum or plasma. This assay employs the quantitative enzyme immunoassay technique. A highly purified Proteinase 3 (PR3) has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any PR3 antibodies (Ab) present is bound by the immobilized antigen. After washing away any unbound substances, a HRP-conjugated anti-human antibody is added to each well and incubate. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of Ab bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm.The concentration of PR3 Ab in the sample is then determined by comparing the O.D of samples to the standard curve. Sample Types: serum, plasma. Range: 10 - 200 U/ml. Sensitivity: 0.5 U/ml. Time: 1 hour ?