Triglyceride Assay Kit provides a simple, reproducible, and sensitive tool for assaying triglycerides in plasma, serum, cell lysates, and tissue homogenate samples. The Triglyceride Colorimetric Assay uses the enzymatic hydrolysis of the triglycerides by lipase to glycerol and free fatty acids. The glycerol released is subsequently measured by a coupled enzymatic reaction system. The glycerol formed in reaction 1 is phosphorylated to glycerol- 3-phosphate in a reaction catalyzed by glycerol kinase. The glycerol-3- phosphate is oxidized by glycerol phosphate oxidase producing dihydroxyacetone phosphate and hydrogen peroxide. Peroxidase catalyzes the redox-coupled reaction of H2O2 with 4-aminoantipyrine (4-AAP) and N-Ethyl-N-(3-sulfopropyl)- m-anisidine (ESPA), producing a brilliant purple color. The absorbance is measured at 540 nm.