The Ubiquitin Detection kit facilitates the fast, effective capture and detection of ubiquitinated proteins from biological samples. The kit utilises a high capacity, high specificity ubiquitin binding matrix together with an easy-to-use purification system for less 'hands on time' and superior performance. Allows purification of mono- and poly-ubiquitinated proteins, independent of chain linkage or length, but not free ubiquitin. Highly adaptable, compatible with samples from a wide range of species and with a broad range of lysis buffers. Analysis by Western blotting or proteomic methods enables identification and assessment of ubiquitinated proteins of interest. Kit contains sufficient ubiquitin matrix to perform up to 20 assays.
Size
20 samples
Concentration
n/a
Applications
1. Capture and detect ubiquitinated proteins and free chains from cell lysates and tissue extracts2. Demonstrate specific proteins are substrates for ubiquitin modification in vivo3. Identify and characterise ubiquitin modified proteins by proteomic analysis4. Investigate role of ubiquitin in particular signalling pathways